Review



z devd fmk zdevd  (Selleck Chemicals)


Bioz Verified Symbol Selleck Chemicals is a verified supplier
Bioz Manufacturer Symbol Selleck Chemicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Selleck Chemicals z devd fmk zdevd
    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Z Devd Fmk Zdevd, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Z-DEVD-FMK/pmc12053523-44-0-7
    Average 94 stars, based on 118 article reviews
    z devd fmk zdevd - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity"

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity

    Journal: Acta Dermato-Venereologica

    doi: 10.2340/actadv.v105.40697

    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or ZDEVD, were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Figure Legend Snippet: Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or ZDEVD, were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).

    Techniques Used: Western Blot, Cell Culture, Solvent, Incubation, Cell Counting

    Related Articles

    Western Blot:

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand-Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity.
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Cell Culture:

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand-Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity.
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Solvent:

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand-Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity.
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Incubation:

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand-Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity.
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Cell Counting:

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand-Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity.
    Article Snippet: Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).Anti-cleaved caspase-3 (rabbit polyclonal, P42574), anti-PAI-1 (rabbit monoclonal, P05121), anti-caspase-1 (rabbit monoclonal, P29466), anti-caspase-8 (rabbit monoclonal, Q14790), anti-NFκB p65 (mouse monoclonal, Q04206), anti-phospho-NFκB p65 (rabbit monoclonal, Q04206), and the Death Receptor Antibody Sampler Kit II (containing anti-Fas, anti-TNFR1, anti-TRAILR1, anti-TRAILR2) were purchased from Cell Signaling Technology (Danvers, MA, USA).. Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).. Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).Lenvatinib was from MedChem Express (Monmouth Junction, NJ, USA); sorafenib from Santa Cruz Biotechnology (Dallas, TX, USA); ketoconazole and itraconazole from Sigma-Aldrich (Saint Louis, MO, USA); sunitinib from Tocris Bioscience (Bristol, UK); regorafenib from Tokyo Chemical Industry (Tokyo, Japan); and capecitabine from Toronto Research Chemicals (Toronto, Canada).



    Similar Products

    zdevd fmk  (MedChemExpress)
    96
    MedChemExpress zdevd fmk
    Zdevd Fmk, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Z-DEVD-FMK/pmc12752754-461-10-12
    Average 96 stars, based on 1 article reviews
    zdevd fmk - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    z devd fmk zdevd  (Selleck Chemicals)
    94
    Selleck Chemicals z devd fmk zdevd
    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Z Devd Fmk Zdevd, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Z-DEVD-FMK/pmc12053523-44-0-7
    Average 94 stars, based on 1 article reviews
    z devd fmk zdevd - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    apoptosis inhibitor api zdevd fmk  (MedChemExpress)
    96
    MedChemExpress apoptosis inhibitor api zdevd fmk
    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Apoptosis Inhibitor Api Zdevd Fmk, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Z-DEVD-FMK/pm40147528-66-13-18
    Average 96 stars, based on 1 article reviews
    apoptosis inhibitor api zdevd fmk - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    zdevd fmk  (R&D Systems)
    95
    R&D Systems zdevd fmk
    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Zdevd Fmk, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Caspase-3+Inhibitor+Z-DEVD-FMK/pm40033051-233-14-15
    Average 95 stars, based on 1 article reviews
    zdevd fmk - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    caspase 3 inhibitor zdevd fmk  (MedChemExpress)
    96
    MedChemExpress caspase 3 inhibitor zdevd fmk
    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Caspase 3 Inhibitor Zdevd Fmk, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Z-DEVD-FMK/10__1016_slash_j__fbio__2024__105388-53-1-6
    Average 96 stars, based on 1 article reviews
    caspase 3 inhibitor zdevd fmk - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    caspase 3 zdevd fmk  (R&D Systems)
    95
    R&D Systems caspase 3 zdevd fmk
    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
    Caspase 3 Zdevd Fmk, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+devd+fmk+zdevd/Caspase-3+Inhibitor+Z-DEVD-FMK/pm39040105-92-10-19
    Average 95 stars, based on 1 article reviews
    caspase 3 zdevd fmk - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or ZDEVD, were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).

    Journal: Acta Dermato-Venereologica

    Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity

    doi: 10.2340/actadv.v105.40697

    Figure Lengend Snippet: Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or ZDEVD, were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).

    Article Snippet: Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).

    Techniques: Western Blot, Cell Culture, Solvent, Incubation, Cell Counting